The Critical Voltage Effect in High-Voltage Electron Microscopy

The Critical Voltage Effect in High-Voltage Electron' Microscopy

High-voltage electron microscopy of normal human cornea.

Conventional transmission electron microscopy (CTEM) was compared with high-voltage electron microscopy (HVEM) on 11 normal human corneas (age range, 30 weeks of gestation to 92 yr). Epithelial anchoring fibrils were noted between the basal epithelial cells and Bowman's layer (BL) as previously reported. Parallel pairs of fibers, 27.5 nm in diameter, were observed crossing into the anterior str...

The High Voltage Electron Microscope in Biology

The first published micrograph of a biological specimen photographed in a high voltage electron microscope (HVEM) appeared in a paper by Van Dorsten et al. in 1947, and it is consequently surprising that the HVEM does not play a greater role in biology 27 yr later. There are various reasons for this. The first prototypes were developed with the aim of enabling the investigator to examine thicke...

Foreword to the special issue low-voltage electron microscopy.

Today, low voltage electron microscopy is a topic of high relevance all over the world; in Japan [1], America [2], and Germany [3] particular research projects are even dedicated to unravel the atomic and electronic structure of radiation sensitive matter with 60 kV electrons and below. It seems that research comes back to its voltage-routes of the early days of electron microscopy, when in 193...

Structural analysis of the Leptospiraceae and Borrelia burgdorferi by high-voltage electron microscopy.

Spirochetes are an evolutionary and structurally unique group of bacteria. Outermost is a membrane sheath (OS), and within this sheath are the protoplasmic cell cylinder (PC) and periplasmic flagella (PFs). The PFs are attached at each end of the PC and, depending on the species, may or may not overlap in the center of the cell. The precise location of the PFs within the spirochetal cells is un...